Tripartite Motif Containing Protein 47 (TRIM47)

The deduced 638-amino acid GOA protein contains an N-terminal ring finger domain, 2 B-box domains, and a coiled-coil domain, placing it in the RBCC (ring finger B-box coiled-coil) protein family. In addition, GOA contains a C-terminal B30.2 domain and 2 LXXLL motifs.GOA shares highest sequence identity with the estrogen-responsive ring finger protein EFP (ZNF147). Northern blot analysis detected a 2.3-kb transcript that was significantly overexpressed in 6 of 7 high-grade astrocytomas and all 3 low-grade astrocytomas examined in comparison with normal brain. In normal tissues GOA was expressed at low levels comparable to those seen in normal brain, with the exception of kidney, in which expression was about 10-fold higher.