Yip1 Interacting Factor Homolog A (YIF1A)

Matern et al. (2000) cloned yeast Yif1, and by database analysis, they identified the human ortholog YIF1A, which encodes a deduced 293-amino acid protein with 5 transmembrane domains in its C-terminal half. Database analysis showed that YIF1A is conserved across species. Fluorescence-tagged YIF1A was expressed with Golgi marker proteins in transfected HeLa cells, and its localization became diffuse during the dissolution of the Golgi apparatus in metaphase.human YIPF5 and YIF1A interact directly. Both proteins localized to the Golgi apparatus in transfected human embryonic kidney cells, and the Golgi localization of YIF1A depended upon the transmembrane domain of YIPF5. Mutation analysis revealed that both the N and C termini of YIF1A were required to anchor YIF1A to Golgi.