Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6)

The deduced 208-amino acid AWP1 protein has a calculated molecular mass of 22.56 kD and shares approximately 55% sequence homology with mouse Awp1 and human ZNF216. AWP1 has 2 conserved zinc finger domains: ZFA20 at its N terminus and ZFAN1 at its C terminus. It contains 2 potential PEST sequences, 4 putative N-glycosylation sites, 7 casein kinase II phosphorylation sites, and 2 N-myristoylation sites. It has a putative nuclear localization signal, but no N-terminal signal peptide, transmembrane domain, or endoplasmic reticulum retention or membrane retention motifs. Northern blot analysis detected ubiquitous expression of a 1.5-kb transcript in all tissues examined, with relatively high levels in heart, skeletal muscle, liver, kidney, and placenta.