Zinc Finger And BTB Domain Containing Protein 32 (ZBTB32)

Sequence analysis predicted that the 487-amino acid protein, which is similar to PLZF, contains an N-terminal POZ/BTB domain and 3 zinc fingers. Northern blot analysis detected weak expression of a 1.4-kb transcript in most tissues tested except peripheral blood leukocytes, where strong expression was detected, and testis, where strong expression of 2.0-, 2.7-, and 4.4-kb transcripts was detected. Coimmunoprecipitation analysis showed that FAZF associates with FANCC independent of the BTB/POZ domain. FANCC and PLZF did not colocalize. EMSA analysis suggested that PLZF and FAZF bind to CpG islands, interact with each other through the POZ domain of PLZF, and repress transcription by a similar mechanism.