Zinc Finger, FYVE Domain Containing Protein 20 (ZFYVE20)

Rabenosyn-5 colocalized with EEA1 on RAB5-positive endosomes in HeLa cells. Mutation analysis indicated that the FYVE domain of rabenosyn-5 was required for endosome targeting. Rabenosyn-5 was complexed with the Sec1-like protein VPS45 (VPS45A), and rabenosyn-5 appeared to serve as a molecular link between VPS45 and RAB5. Both EEA1 and rabenosyn-5 were required for early endosomal fusion, but only rabenosyn-5 overexpression inhibited processing of procathepsin D to its 47-kD intermediate, suggesting that the 2 proteins play distinct roles in endosomal trafficking.The deduced 784-amino acid protein contains an N-terminal C2H2-type zinc finger domain, a central FYVE finger domain, and 5 copies of the amino acid motif NPF at its C terminus.