Zinc Finger, FYVE Domain Containing Protein 27 (ZFYVE27)

Transient transfection of HeLa cells with epitope-tagged ZFYVE27 constructs revealed that ZFYVE27 is predominantly expressed in punctate vesicles, although a tubular pattern of expression was observed in a small proportion of cells.Colocalization studies detected overlapping expression with the endosomal marker EEA1 and the endoplasmic reticulum marker RTN1. Coimmunoprecipitation studies in NIH3T3 cells determined that spastin mediates interaction with ZFYVE27 through its N-terminal domain, which consists of an MIT (microtubule-interacting and trafficking) motif. An immunoprecipitation assay confirmed the interaction of endogenous spastin with ZFYVE27.