Zinc Finger Protein 17 (ZNF17)

The cDNA ZNF-17 was casually isolated from an adult worm lgt11 cDNA library during attempts to obtain the complete cDNA for Sm13, a S. mansoni tegumental antigen. This cDNA was amplified and cloned into pBlueScript KS+, and DNA sequencing revealed that the 603 bp cDNA shared no significant homology to the A157 clone  of the Sm13 gene. On the basis of the sequence of the ZNF-17 cDNA, several primers were sinthesized to sequence the complete open reading frame of the gene and detect the existence of possible introns. The 446 bp fragment SmZU-5R amplified from a lZAP adult worm cDNA library using primers ZnFUpper and ZFR1 allowed for the identification of an additional sequence to the 5""" region, while the 349 bp fragment UUEE-3F amplified from another lZAP adult worm cDNA library with primers ZFF1 and ZnFLower, identified the poly-A tail of the 3""" region of the gene.