Zinc Finger Protein 200 (ZNF200)

ZNF200 contains 5 tandem Kruppel-like C2H2 domains, each of which binds a zinc ion to form a globular domain. Western blot analysis detected endogenous ZNF200 at an apparent molecular mass of 50 kD in human embryonic kidney cells. Epitope-tagged ZNF200 colocalized with G9A (EHMT2) in most areas of the nucleus as discrete speckles. During metaphase, the 2 proteins colocalized outside of mitotic chromosomes.During genomic sequencing and exon trapping in the chromosomal region around the familial Mediterranean fever locus (MEFV), the French FMF Consortium (1997) identified a novel gene encoding a zinc finger protein. The authors amplified all 5 exons from a human liver cDNA library. The open reading frame encodes a 394-amino acid polypeptide.