Zinc Finger Protein 219 (ZNF219)

ZNF219 bound to potential ZNF219-binding sites in the HMGN1 promoter and repressed transcription from the HMGN1 promoter in a reporter assay. A proline-rich sequence was responsible for part of the repression activity.The deduced 723-amino acid ZNF219 protein contains 9 sets of Kruppel-related zinc finger structures and several proline-rich regions. Northern blot analysis detected ZNF219 expression in all fetal and adult tissues tested. The ZNF219 transcript was 5.5 kb in adult tissues, whereas a predominant 3.5-kb transcript as well as the 5.5-kb transcript was detected in fetal tissues. When a plasmid cloned with green fluorescent protein-tagged ZNF219 was expressed in HeLa and COS-7 cells, strong fluorescence intensities were observed only in the nucleus of both types of cells by confocal microscopy.