Zinc Finger Protein 224 (ZNF224)

The deduced 623-amino acid ZNF224 protein contains 18 tandemly repeated C-terminal zinc finger motifs with interspersed conserved knuckle sequences, as well as an N-terminal Kruppel-related novel box (KRNB), which is also found in FDZF2 (ZNF230). RT-PCR analysis detected wide expression that was highest in hemopoietic tissues and cell lines.The deduced 622-amino acid protein has a calculated molecular mass of 72 kD. Northern blot analysis detected expression only in fetal tissues. Transcripts of about 5.0 and 4.0 were detected in fetal brain, lung, liver, and kidney, and a transcript of about 3.4 kb was also detected in fetal lung. Western blot analysis of cytosolic and nuclear fractions obtained from transfected cells indicated that ZNF224 is expressed in the nucleus.