Zinc Finger Protein 24 (ZNF24)

Using PCR primers based on conserved sequences of Kruppel-like genes to screen an acute promyelocytic leukemia cell line, Han et al. (1999) isolated cDNAs encoding ZNF24, which they termed ZNF191. The deduced 368-amino acid protein contains 4 tandemly repeated C-terminal zinc finger motifs with interspersed conserved knuckle sequences, as well as an N-terminal SCAN box, which is enriched in hydrophobic and negatively charged residues and is also found in ZNF174 and ZNF165. Northern blot and RT-PCR analyses revealed expression of 6.0- and 2.5-kb transcripts in all tissues tested except heart. By functional analysis, Han et al. (1999) showed that the ZNF191 SCAN box has no activity in yeast but acts as a transrepressor in mammalian cell lines.