Zinc Finger Protein 396 (ZNF396)

ZNF396 encodes a full-length protein of 335 amino acids with an N-terminal SCAN domain and 3 tandem C-terminal C2H2 zinc finger repeats. Alternative splicing produces a truncated protein of 210 amino acids that lacks the zinc fingers. Both ZNF396 proteins share an identical N-terminal region of 187 amino acids containing 1 SCAN domain. The full-length ZNF396 protein shares 57% amino acid identity with ZNF24.Northern blot analysis detected ZNF396 transcripts of 3.9, 2.5, and 1.5 kb. Expression was strong in liver, moderate in skeletal muscle, and weak in kidney, pancreas, spleen, and prostate. Immunohistochemistry showed that full-length ZNF396 protein localized to the nucleus and truncated ZNF396 protein localized diffusely throughout the cell.