Zinc Finger Protein 461 (ZNF461)

By large-scale sequence analysis of a fetal brain cDNA library, Dai et al. (2003) cloned ZNF461. The deduced 563-amino acid protein has a calculated molecular mass of 66 kD. ZNF461 contains a double KRAB domain at the N terminus and 11 C2H2-type zinc finger motifs at the C terminus, suggesting a role in DNA binding. It shares 68% amino acid identity with rat Giot1. RT-PCR detected ubiquitous expression, with highest levels in liver, kidney, pancreas, thymus, and small intestine.Dai et al. (2003) determined that the ZNF461 gene contains 6 exons and spans about 26 kb.A zinc finger protein is a DNA-binding protein domain consisting of zinc fingers ranging from two in the Drosophila regulator ADR1, the more common three in mammalian Sp1 up to nine in TFIIIA.