Zinc Finger Protein 496 (ZNF496)

ZNF496 contains a SCAN box, a KRAB-A domain, and four consensus C2H2-type zinc fingers preceded by a unique finger derivative, referred to herein as the C2HR motif. The C2HR motif functions to mediate protein-protein interaction with the cysteine-rich (C5HCH) domain of NSD1 in a Zn(II)-dependent fashion, and when tethered to RNA polymerase II promoters, represses transcription in an NSD1-dependent manner. ZNF496 contains a novel type of zinc finger motif that functions as a docking site for NSD1 and is more than just a degenerate evolutionary remnant of a C2H2 motif. The gene contains 16 different gt-ag introns. Transcription produces 13 different mRNAs, 11 alternatively spliced variants and 2 unspliced forms. There are 4 probable alternative promotors, 5 non overlapping alternative last exons and 8 validated alternative polyadenylation sites .