Zinc Finger Protein 513 (ZNF513)

ZNF513 associated with the proximal promoters of the retinal transcription factors Pax6 and Sp4 and those of their targets rhodopsin (RHO), Irbp (RBP3), Arr3, and photoreceptor opsins.The deduced 541-amino acid protein has 2 N-terminal nuclear localization signals and 3 C2H2-type zinc finger motifs. Quantitative RT-PCR detected ubiquitous expression in mouse. In mouse ocular tissues, expression was highest in retina and lowest in lens and cornea. Retinal expression of mouse Znf513 increased progressively with age and began to level off between 180 and 300 days. In situ hybridization of human retina detected ZNF513 in outer nuclear layer, inner nuclear layer, and ganglion cell layer. Fluorescence-tagged ZNF513 was expressed in dispersed nuclear foci, but not in nucleolus, of transfected COS-7 cells.