Zinc Finger Ran Binding Domain Containing Protein 1 (ZRANB1)

By searching an EST database for sequences similar to A20 (TNFAIP3), followed by RACE PCR of human umbilical vein endothelial cell cDNA, Evans et al. (2001) cloned ZRANB1, which they called TRABID. The deduced protein contains 3 N-terminal nucleoporin (see NUP98)-type zinc fingers and a C-terminal TRAF (see TRAF1)-binding domain. Northern blot analysis detected major 3-kb and minor 1-kb transcripts in human epithelial cells and fibroblasts and in a liver carcinoma cell line. Fluorescence-tagged TRABID distributed diffusely in the cytoplasm and nuclei of all cell lines tested and accumulated in nuclei of most fibroblasts.Evans et al. (2001) showed that exogenous A20, Cezanne (OTUD7B) and TRABID coimmunoprecipitated with TRAF6 when they were overexpressed in human embryonic kidney cells.