Lysine Specific Demethylase 3B (KDM3B)

KDM3B shares significant similarity with rat Tsga (JMJD1A), a protein expressed only in testis. RT-PCR ELISA detected moderate to high expression in all adult and fetal tissues and specific brain regions examined.C5ORF7 gene contains 24 exons and spans about 64 kb.By EST analysis, screening a bone marrow cDNA library, 5-prime RACE, and RT-PCR, they cloned C5ORF7. The deduced 1,417-amino acid protein has a calculated molecular mass of about 155 kD. It contains a putative zinc finger domain and a bipartite nuclear localization signal. Northern blot analysis of lymphoid tissues detected a transcript of about 7.5 kb. RNA dot blot analysis detected expression in all 50 tissues examined.