Phosphohistidine Phosphatase 1 (PHPT1)

PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I . PHPT1 gene contains 3 exons.The deduced 125-amino acid PHPT1 protein migrated as a single, approximately 14 kD band by SDS-PAGE. Northern blot analysis detected a 0.6-kb transcript, with highest expression in heart and skeletal muscle and lower expression in several other tissues including pancreas, liver, and kidney.recombinant PHPT1 overexpressed and purified from bacteria displayed specific phosphohistidine phosphatase activity toward phosphopeptide I. No activity was detected towards phosphoserine, phosphothreonine, or phosphotyrosine peptides.