Immunoglobulin Lambda 2 (Igl2)

Immunoglobulin lambda locus is a region on human chromosome 22 that contains genes for the lambda light chains of antibodies (or immunoglobulins). Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus. The locus includes V (variable), J (joining), and C (constant) segments. The PCR products of the antigen binding exons of the chicken IgL and IgH chains then were cloned into expression vectors that contained the murine Igλ2 light chain gene or the murine IgGγ1 heavy chain gene. These expression plasmids had been modified to have directional cloning sites that allowed the direct replacement of the mouse VJ or VDJ by the chicken VJ or VDJ segment.