Angiopoietin Like Protein 2 (ANGPTL2)

The predicted 493-amino acid ANGPTL2 protein contains the coiled-coil and fibrinogen-like domains that are conserved in angiopoietins. ANGPTL2 also has a putative N-terminal secretory signal sequence and 2 potential glycosylation sites. Human ANGPTL2 shares 95% amino acid sequence identity with mouse Angptl2, 59% identity with human ANGPTL1, 35% identity with mouse Angpt3 and human ANGPT4, and 34% identity with human ANGPT1 and ANGPT2. Recombinant ANGPTL2 expressed in mammalian cells was secreted and glycosylated. By Western blot analysis, recombinant ANGPTL2 had a molecular mass of approximately 64 kD. Northern blot analysis of human adult tissues detected abundant expression of a major 4.0- and a minor 2.6-kb ANGPTL2 transcript in heart, small intestine, spleen, and stomach.