Hepatocellular Carcinoma Related Protein 1 (HCRP1)

HCRP1 encodes a deduced 397-amino acid protein. Northern blot analysis showed ubiquitous expression of an approximately 2-kb HCRP1 transcript, with most abundant expression in liver. Western blot analysis showed reduced HCRP1 expression in 6 of 8 HCC tissues, but higher expression levels of HCRP1 in adjacent normal tissues in all 8 cases. Transient transfection of GFP-HCRP1 fusion constructs showed that HCRP1 is localized throughout the cell, but predominantly in the nucleus.Pull-down assays demonstrated that the interaction between HCRP1 and TSG101 occurs through the mod(r) domain. By size exclusion chromatography, HCRP1 cofractionated with TSG101 and VPS28. Depletion of TSG101 by siRNA treatment resulted in reduction in HCRP1 levels in HeLa cells.