Active Amiloride Binding Protein 1 (ABP1) (APA656Hu61)

Figure. The binding activity of DAO with DDC. Diamine oxidase (DAO), also known as histaminase, is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. It is also secreted by eosinophils. In case of a shortage of diamine oxidase in the human body, it may appear as an allergy or histamine intolerance. Besides, Dopa Decarboxylase (DDC) has been identified as an interactor of DAO, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DAO and recombinant human DDC. Briefly, DAO were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to DDC-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DAO pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of DAO and DDC was shown in Figure 1, and this effect was in a dose dependent manner.

Figure. Gene Sequencing (extract)

Figure. SDS-PAGE