Active C Reactive Protein (CRP) (APA821Ra01)

C-reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q.Besides,CRP have been proved can promote migration of HepG2 cells, 5×104 cells were seeded into 6 well plates. After cell confluent, using a (yellow) pipette tip make a straight scratch, simulating a wound, then washing the wells three times with PBS. Adding 1% serum standard DMEM containing various concentrations of recombinan rat CRP to each well, incubating the plate for 48 hours at 37℃, 5% CO2. Use Image J to measure the area of a scratch, then caculate the cell motility with (0harea -48harea)/0harea ×100%. After affect with 62.5ng/ml CRP for 48h, cell motility is 47%,wiithout affect by CRP, the cell motility is 27%. The results observed by inverted microscope was shown in Figure1.