Active Catalase (CAT) (APC418Hu05)

Catalase (CAT) is an antioxidant enzyme present in all aerobic organisms. It is known to catalyze H2O2 into water and oxygen in an energy-efficient manner in the cells exposed to environmental stress. As we know, H2O2 and (NH4)2MoO4 can produce a stable yellow product which has a absorbance at 405 nm. Thus, the recombinant human CAT activity was measured by its ability to hydrolyze H2O2 to H2O , the remaining H2O2 in the reaction was measured by (NH4)2MoO4. The reaction was performed in 60 mM phosphate buffer pH 7.4 (Assay Buffer), initiated by addition 100 μL of various concentrations of CAT (diluted by Assay Buffer) to 1 mL of 65 mM Substrate H2O2 (diluted by Assay Buffer). Incubated at 37℃ for 1min, then add 1 ml 200 mM (NH4)2MoO4. After 10 minutes at room temperature, read at a wavelength of 405 nm.