Active Interferon Gamma (IFNg) (APA049Gu01)

Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. It has been reported that IFN-γ promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages as an important activator. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 264.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 264.7 cells were incubated in DMEM with IFN-γ (10ng/mL) for 24h, then cells were observed by inverted microscope and iNOS in cell lysates was detected by ELISA. Effect of IFN-γ on morphological change of Raw 246.7 cells was shown in Figure 1.

Effect of IFN-γ on the expression of iNOS was shown in Table 1.

Figure. Gene Sequencing (extract)

Figure. Western Blot; Sample: Recombinant IFNg, Cavia.

Figure. SDS-PAGE