Active Interleukin 6 (IL6) (APA079Po01)

Figure. Cell proliferation of Raji cells after stimulated with IL6. Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 which contains various concentrations of recombinant pig IL6. After incubated for 5 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of Raji cells after incubation with IL6 for 5 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant IL6 for 5 days. The result was shown in Figure 2. It was obvious that IL6 significantly increased cell viability of Raji cells. (A) Raji cells cultured in 1640, stimulated with 10ng/mL IL6 for 5 days; (B) Unstimulated Raji cells cultured in 1640 for 5 days.

Figure. Cell proliferation of Raji cells after stimulated with IL6.

Figure. Western Blot

Figure. Gene Sequencing (extract)

Figure. SDS-PAGE