Active Lipopolysaccharide Binding Protein (LBP) (APB406Mu02)

Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4.This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). It has been reported that LBP can enhance LPS-stimulated IL-8 secretion by THP-1 cells. To test the bioactivity of LBP, THP-1 cells were seeded into 24-well plate at a density of 1x106 cells/mL including 1ug/mL LPS, and treated with certain concentrations (0.625ug/mL-20ug/mL) of LBP for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of THP-1 cells increased significantly after stimulated with LBP have shown in Figure1.