Active Secondary Lymphoid Tissue Chemokine (SLC) (APB575Hu01)

Secondary Lymphoid-tissue Chemokine (SLC) is a recently identified CC chemokine that is constitutively expressed in various lymphoid tissues and is a potent and specific chemoattractant for lymphocytes. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of SLC on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100uL cell suspension,106 cells/mL in RPMI 1640 with FBS free) and SLC (1ng/mL, 15ng/mL and 150ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows SLC is able to induce migration of Jurkat cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of SLC occurs at 15-150ng/mL.(A)Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 with 150ng/mL SLC was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h; (B)Jurkat cells were seeded into the upper chambers and serum free RPMI 1640 without SLC was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h. Figure. The chemotactic effect of SLC on Jurkat cells

Figure.The chemotactic effect of SLC on Jurkat cells.

Figure. Gene Sequencing (extract)

Figure. Western Blot

Figure. Gene Sequencing (extract)

Figure. SDS-PAGE