Active Tumor Necrosis Factor Alpha (TNFa) (APA133Mu01)

Figure 1. Cell apoptosis of A549 cells after stimulated by TNFa. ;TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β in cell supernatant was detected by ELISA. Cell apoptosis of A549 after incubation of 48h was shown in Figure 1. ; (A)A549 cells cultured in DMEM, stimulated with 1ng/mL TNFa for 48h; ;(B)A549 cells cultured in DMEM, stimulated with 10ng/mL TNFa for 48h; ;(C)A549 cells cultured in DMEM for 48h.

Figure. Gene Sequencing (extract)

Western Blot, Sample: APA133Mu01; Antibody: PAA133Mu01

Table 1. ELISA detection of IL-1β expression from A549 cells by TNFa. Effect of TNFa on the expression of IL-1β is shown in Table 1.

Figure. SDS-PAGE