Collagen III Mouse mAb (BF9208)

Western blot analysis of Hela with Collagen III Mouse mAb diluted at 1:1,000.

BF9208 at 1/100 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ºС. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.

BF9207 staining MCF cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-mouse IgG (H+L) Antibody (Red) diluted at 1/600, was used as secondary antibody.