Acetyl-Histone H2A (Lys9) Antibody (DF2929)

Western blot analysis of extracts from various samples, using Acetyl-Histone H2A (Lys9) Antibody. Lane 1: P19 cells (heat shock treatment) blocked with antigen-specific peptides, Lane 2: P19 cells (heat shock treatment), Lane 3: HepG2 cells (heat shock treatment).

DF2929 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22ºС. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

DF2929 staining Hela cells (heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (DF2929) and mouse anti-beta tubulin Ab (T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).