Acetyl-Histone H2B (Lys20)? Antibody (AF4361)

Western blot analysis of Acetyl-Histone H2B (K20) in lysates of NIH/3T3 cells (TSA 1M, 18 hr) using Acetyl-Histone H2B (K20) Antibody (AF4361).

AF4361 at 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

AF4361 staining HepG2 cells (30 min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (AF4361) and mouse anti-beta tubulin Ab (T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).