ADRA1A Antibody (DF4852)

Western blot analysis of ADRA1A Antibody expression in mouse kidney tissue lysates. The Lane on the right was treated with the antigen-specific peptide.

DF4852 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

DF4852 staining HepG2 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37ºС. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37ºС. A AlexaFluor594 conjugated goat polyclonal to rabbit IgG (H+L) diluted 1/600 was used as secondary antibody.