ARIH2 Antibody (DF12832)

Western blot analysis of extracts from HeLa cells, using ARIH2 Antibody. The Lane on the left was treated with blocking peptide.

DF12832 at 1/100 staining human pancreatic cancer and para-carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.

DF12832 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Antibody (Red) diluted at 1/600, was used as secondary antibody.