Bcl-2 Antibody (BF9103)

Western blot analysis of extracts from various samples, using Bcl-2 Antibody. Lane 1: HepG2 cells Lane 2: mouse testis tissue lysate Lane 3: rat brain tissue lysate Lane 4: mouse brain tissue lysate Lane 5: RAW 264.7 cells heat-shocked Lane 6: LOVO cells Lane 7: C6 cells serum-starved Lane 8: 3T3 UV cells Lane 9: EC304 cells

BF9103 at 1/100 staining Mouse ovary tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ºС. An HRP conjugated goat anti-Mouse antibody was used as the secondary antibody.

BF9103 staining Jurkat cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (BF9103 1:200) and Rabbit anti-beta tubulin Ab (AF7011 1:200) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-Mouse IgG (H+L) Ab (Red) and an AlexaFluor 488 conjugated goat anti-Rabbit IgG (H+L) Ab (Green) were used as the secondary antibody.