Bub1 Antibody (AF6768)

Western blot analysis of extracts from various samples, using Bub1 Antibody. Lane 1: Hela cells (heat shock treatment) blocked with antigen-specific peptides, Lane 2: Hela cells (heat shock treatment), Lane 3: HepG2 cells (serum starvation treatment).

AF6768 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (AF6768) and mouse anti-beta tubulin Ab (T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).