Calpastatin Antibody (DF13778)

Western blot analysis of extracts from various samples, using Calpastatin Antibody. Lane 1: Rat liver, blocked with antigen-specific peptides. Lane 2: Rat liver. Lane 3: Mouse brain. Lane 4: HepG2 cells (heat-shock treatment). Lane 5: Hela cells (heat-shock treatment).

DF13778 at 1/100 staining human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.

DF13778 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (#DF13778) and mouse anti-beta tubulin Ab (#T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).