CBLN2 Antibody (DF3924)

Western blot analysis of extracts from various samples, using CBLN2 Antibody. Lane 1: PC12 (H2O2 treatment) blocked with antigen-specific peptides, Lane 2: PC12 (H2O2 treatment), Lane 3: RAW264.7 cells (heat shock treatment).

DF3924 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (DF3924) and mouse anti-beta tubulin Ab (T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).