CCNT2 Antibody (DF2289)

Western blot analysis of extracts from HepG2 cells (H2O2 treatment) using CCNT2 Antibody. The Lane on the left was treated with blocking peptide.

DF2289 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (DF2289) and mouse anti-beta tubulin Ab (T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).