Cleaved-Caspase 3 (Asp175); p17 Antibody (AF7022)

Western blot analysis of extracts from various samples, using Cleaved Caspase 3 Antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela (etoposide treated, 25uM 5h); Lane 3: MCF7 (etoposide treated, 25uM 5h); Lane 4: Mouse heart; Lane 5: Mouse spleen.

AF7022 at 1/100 staining human ehrlich carcimoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22ºС. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

AF7022 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (AF7022 1:200) and mouse anti-beta tubulin Ab (T0023 1:200) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).