Cleaved-Caspase 9 (Asp315) Antibody (AF4000)

Western blot analysis of extracts from MCF7, using Cleaved Caspase-9 (Asp315) Antibody. The Lane on the left was treated with blocking peptide. Observed bands: 45 kDa.

AF4000 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (AF4000 1:200) and mouse anti-beta tubulin Ab (T0023 1:200) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).