Cytokeratin 14 Antibody (AF5370)

Western blot analysis of Keratin 14 expression in NIH/3T3 cells

AF5370 at 1/100 staining Human colon cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22ºС. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

AF5370 staining sw480 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Antibody (Red) diluted at 1/600, was used as secondary antibody.