EFNA1 Antibody (DF7284)

Western blot analysis of Hela whole cell lysates, using EFNA1 Antibody. The Lane on the left was treated with the antigen-specific peptide.

DF7284 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

DF7284 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Antibody (Red) diluted at 1/600 was used as secondary antibody.