FGF8 Antibody (DF2497)

Western blot analysis of extracts from various samples, using FGF8 Antibody. Lane 1: Myeloma cells, treated with blocking peptide. Lane 2: Myeloma cells; Lane 3: Hela cells; Lane 4: Mcf7; Lane 5: B16F10 cells. Observed bands: 20 kDa.

DF2497 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (DF2497 1:200) and mouse anti-beta tubulin Ab (T0023 1:200) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).