Histone H2B antibody (BF0722)

Western blot analysis of extracts from various samples, using Histone H2B mouse monoclonal antibody. Lane1: K562, Lane2: PC12, Lane3: mouse brain, Lane4: rat brain

BF0722 at 1/100 staining Human gastric cancer and para-carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-Mouse antibody was used as the secondary antibody.

BF0722 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (BF0722) and rabbit anti-beta tubulin Ab (AF7011) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-mouse IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).