Histone H3 Antibody (BF9211)

Western blot analysis of with Histone H3 Mouse mAb diluted at 1:5,000.

BF9211 at 1/100 staining Human prostate cancer and para-carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-Mouse antibody was used as the secondary antibody.

BF9211 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (BF9211) and rabbit anti-beta tubulin Ab (AF7011) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-mouse IgG (H+L) Ab (Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG (H+L) Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).