IgA Antibody (DF8563)

Western blot analysis of extracts from various samples, using IgA Antibody. Lane 1: Rat lung, blocked with antigen-specific peptides, Lane 2: Rat lung, Lane 3: HepG2 cells.

DF8563 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (#DF8563) and mouse anti-beta tubulin Ab (#T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).