IMMP2L Antibody (DF9630)

Western blot analysis of extracts from various samples, using IMMP2L Antibody. Lane 1: 3t3 treated with blocking peptide. Lane 2: 3t3; Lane 3: Mouse muscle.

DF9630 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

DF9630 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG (H+L) Antibody (Red) diluted at 1/600 was used as secondary antibody.