MMP8 Antibody (AF5446)

Western blot analysis of MMP-8 expression in NIH/3T3 cells. The Lane on the left was treated with the antigen-specific peptide.

AF5446 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

AF5446 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37ºС. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37ºС. A Alexa Fluor594 conjugated goat polyclonal to rabbit IgG (H+L) diluted 1/600 was used as secondary antibody.