| UNIPROT | Q96P11 |
| Иммуноген | Синтетический пептид, соответствующий human NSUN5 |
NSUN5 Antibody (DF15116)
Реактивность подтвержденная:
человек, мышь
Источник (хозяин):
кролик
Валидировано для:
IHC
WB
IF
ICC
Валидация Affinity DF15116 - 11363
Western blot analysis of extracts from various samples, using NSUN5 Antibody. Lane 1: HepG2 cells (heat-shock treatment) blocked with antigen-specific peptides. Lane 2: HepG2 cells (heat-shock treatment). Lane 3: Hela cells (heat-shock treatment).
Валидация Affinity DF15116 - 11364
DF15116 at 1/100 staining mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
Валидация Affinity DF15116 - 11365
DF15116 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (#DF15116) and mouse anti-beta tubulin Ab (#T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).
Характеристики
Техническое описание
| Каталожный номер | DF15116 |
| Название антигена | NOP2/Sun Domain Family, Member 5 (NSUN5) |
| UNIPROT | Q96P11 |
| Иммуноген | Синтетический пептид, соответствующий human NSUN5 |
| Английские синонимы | WBSCR20, WBSCR20A, NOL1R, p120, NSUN5A, Williams Beuren Syndrome Chromosome Region 20A, NOL1/NOP2/Sun Domain Family, Member 5, NOL1-related protein |
Реактивность подтвержденная Нужна другая реактивность? |
человек, мышь |
| Источник (хозяин) | кролик |
| Изотип | IgG |
| Клональность | поликлональное |
| Рекомбинантное | нет |
| Готовое к использованию | нет |
| Концентрация | 1 мг/мл |
| Конъюгат | нет |
| Валидировано для | IHC WB IF ICC Иммуногистохимия; Вестерн-блоттинг; Иммунофлуоресценция; Иммуноцитохимия |
| Рекомендуемые разведения | IF/ICC: 1:100-1:500; IHC: 1:50-1:200; WB: 1:500-1:2000; Оптимальное разведение определяется конечным потребителем |
| Метод очистки | Антисыворотку очищали методом аффинной хроматографии пептидов с использованием связывающей смолы SulfoLink® (Thermo Fisher Scientific) |
| Состав буфера | Rabbit IgG в PBS; pH 7.4; 150mM NaCl; 0.02% азид натрия и 50% глицерин |
| Условия доставки | Термобокс с аккумуляторами холода |
| Условия хранения | Хранить при -20ºС. Стабилен 12 месяцев с даты получения |
| Срок хранения | 12 месяцев |
| Производитель | Affinity |
| Ссылка на страницу товара на сайте производителя | https://www.affbiotech.com/goods-19560-DF15116-NSUN5_Antibody.html |
Валидация Affinity DF15116 - 11363
Western blot analysis of extracts from various samples, using NSUN5 Antibody. Lane 1: HepG2 cells (heat-shock treatment) blocked with antigen-specific peptides. Lane 2: HepG2 cells (heat-shock treatment). Lane 3: Hela cells (heat-shock treatment).
Валидация Affinity DF15116 - 11364
DF15116 at 1/100 staining mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4ºС overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
Валидация Affinity DF15116 - 11365
DF15116 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100. Then blocked in 10% serum for 45 minutes at 25ºС. Samples were then incubated with primary Ab (#DF15116) and mouse anti-beta tubulin Ab (#T0023) for 1 hour at 37ºС. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab (Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).
|
Артикул
|
Наименование |
Производитель
|
|---|
Акции и предложения
Популярные продукты:
Bovine AP 3 complex subunit mu 2(AP3M2) ELISA kit (E11A1572)
Организм: корова
Rat Tubulin alpha-8 chain ELISA kit (E02T0947)
Организм: крыса
С нами успешно сотрудничают:
Московский государственный университет им. Ломоносова
Московский государственный университет им. Ломоносова
